University of Oklahoma, Norman View Institution's Website 10 articles published in JoVE Environment In Situ Soil Moisture Sensors in Undisturbed Soils Todd G. Caldwell1, Michael H. Cosh2, Steven R. Evett3, Nathan Edwards4, Heather Hofman5, Bradley G. Illston6, Tilden Meyers7, Marina Skumanich8, Kent Sutcliffe9 1Nevada Water Science Center, U.S. Geological Survey, 2Agricultural Research Service, Hydrology and Remote Sensing Lab., U.S. Department of Agriculture, 3Agricultural Research Service, Conservation and Production Research Lab., U.S. Department of Agriculture, 4South Dakota State University, 5National Water and Climate Center, U.S. Department of Agriculture, 6University of Oklahoma, 7Air Resources Lab, National Oceanic and Atmospheric Administration, 8National Integrated Drought Information System, National Oceanic and Atmospheric Administration, 9Snow Survey Staff, U.S. Department of Agriculture The determination of soil water content is a critical mission requirement for many state and federal agencies. This protocol synthesizes multi-agency efforts to measure soil water content using buried in situ sensors. Environment Simulating Temperature in a Soil Incubation Experiment Jianwei Li1, Precious Areeveso1, Xuehan Wang1, Siyang Jian1,2, Lahiru Gamage1 1Department of Agricultural and Environmental Sciences, Tennessee State University, 2Department of Plant Biology and Microbiology, University of Oklahoma, Norman Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation studies. Immunology and Infection Chemical Cartography Approaches to Study Trypanosomatid Infection Danya A. Dean*1,2, Jacob J. Haffner*2,3, Mitchelle Katemauswa1, Laura-Isobel McCall1,2,4 1Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 2Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, 3Department of Anthropology, University of Oklahoma, Norman, 4Department of Microbiology and Plant Biology, University of Oklahoma, Norman This protocol describes the steps to generate a 3D model of metabolite distribution during trypanosomatid infection, including sample collection, metabolite extraction, an overview of liquid chromatography-tandem mass spectrometry data acquisition, 3D model generation, and finally, data visualization. Chemistry Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells Shawna J. Standke1, Devon H. Colby1, Ryan C. Bensen1, Anthony W. G. Burgett1, Zhibo Yang1 1Department of Chemistry and Biochemistry, University of Oklahoma An integrated cell manipulation platform is developed for use in conjunction with a single-probe mass spectrometry setup for the on-line analysis of individual suspension cells under ambient conditions. Bioengineering Biaxial Mechanical Characterizations of Atrioventricular Heart Valves Colton Ross*1, Devin Laurence*1, Yi Wu1, Chung-Hao Lee1,2 1Biomechanics and Biomaterials Design Laboratory (BBDL), School of Aerospace and Mechanical Engineering, The University of Oklahoma, 2Institute for Biomedical Engineering, Science and Technology (IBEST), The University of Oklahoma This protocol involves characterizations of atrioventricular valve leaflets with force-controlled, displacement-controlled, and stress-relaxation biaxial mechanical testing procedures. Results acquired with this protocol can be used for constitutive model development to simulate the mechanical behavior of functioning valves under a finite element simulation framework. Engineering Magnet Assisted Composite Manufacturing: A Flexible New Technique for Achieving High Consolidation Pressure in Vacuum Bag/Lay-Up Processes Maya Pishvar*1, Mehrad Amirkhosravi*1, M. Cengiz Altan1 1School of Aerospace and Mechanical Engineering, University of Oklahoma A new technique for applying consolidation pressure on the vacuum bag lay-up to fabricate composite laminates is described. The goal of this protocol is to develop a simple, cost-effective technique to improve the quality of laminates fabricated by the wet lay-up vacuum bag method. Bioengineering A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering Christopher A. Neal1, Jennifer G. Nelson-Brantley1, Michael S. Detamore2, Hinrich Staecker1, Adam J. Mellott3 1Department of Otolaryngology, University of Kansas Medical Center, 2Stephenson School of Biomedical Engineering, University of Oklahoma, 3Department of Plastic Surgery, University of Kansas Medical Center The goal of this protocol is to demonstrate an effective method to decellularize and decalcify mouse cochleae for utilization as scaffolds for tissue engineering applications. Biology Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions Wei Rao1, Ning Pan1, Zhibo Yang1 1Department of Chemistry and Biochemistry, University of Oklahoma Here, we present protocols to perform both ambient mass spectrometry imaging (MSI) of tissues and in-situ live single cell MS (SCMS) analysis using the single-probe, which is a miniaturized multifunctional device for MS analysis. Neuroscience Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS Taylor R. Fore1, Audrey A. Ojwang1, Margaret L. Warner1, Xinyun Peng1, Rudolf A. Bohm1,2, William P. Welch1, Lindsey K. Goodnight1, Hong Bao1, Bing Zhang1 1Department of Zoology, University of Oklahoma - Norman, 2Department of Biology, Brandeis University We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping. Neuroscience Electrophysiology of Scorpion Peg Sensilla Elizabeth D. Knowlton1, Douglas D. Gaffin1 1Department of Zoology, University of Oklahoma This article describes an electrophysiological method for isolating chemical stimulation to individual sensilla via extracellular, tip-recordings under mineral oil.
