Measurement of Heme Synthesis Levels in Mammalian Cells

Jagmohan Hooda; Maksudul Alam; Li Zhang

doi:10.3791/51579

Journal

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생물학

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포유 동물 세포에서 헴 합성 레벨 측정

Measurement of Heme Synthesis Levels in Mammalian Cells

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JoVE 신문 생물학

Measurement of Heme Synthesis Levels in Mammalian Cells

포유 동물 세포에서 헴 합성 레벨 측정

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12,072 Views

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09:43 min

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July 09, 2015

DOI:

10.3791/51579-v

DOI:

10.3791/51579-v

•

09:43 min

July 09, 2015

•

12033 Views

Jagmohan Hooda¹, Maksudul Alam¹, Li Zhang¹

¹Department of Molecular and Cell Biology, Center for Systems Biology,University of Texas at Dallas

Summary

Altered intracellular heme levels are associated with common diseases such as cancer. Thus, there is a need to measure heme biosynthesis levels in diverse cells. The goal of this protocol is to provide a fast and sensitive method to measure and compare the levels of heme synthesis in different cells.