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Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
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Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development

Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development

14,709 Views

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06:46 min

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September 04, 2014

DOI:

10.3791/51817-v

DOI:
10.3791/51817-v

•

06:46 min
September 04, 2014

•

2 Views
,
1Cell and Developmental Biology,University of Michigan, 2Department of Biosciences and Nutrition,Karolinska Instituet Novum

Summary

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Improved imaging technology is allowing three-dimensional imaging of organs during development. Here we describe a whole organ culture system that allows live imaging of the developing villi in the fetal mouse intestine.

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Mouse Fetal IntestineEx Vivo Culture3D Live ImagingVillus DevelopmentSignaling PathwaysEpithelium MorphogenesisTwo-photon ImagingPseudostratified Epithelium

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Walton, K. D., Kolterud, Å. Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development. J. Vis. Exp. (91), e51817, doi:10.3791/51817 (2014).

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