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Neuroscience

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In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
Journal (Neuroscience)
In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
Middle Cerebral Artery Occlusion (MCAO) to Induce Stroke in a Murine Model (Video) | JoVE
Journal (Neuroscience)
Middle Cerebral Artery Occlusion (MCAO) to Induce Stroke in a Murine Model (Video) | JoVE
 
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Detektion

Article DOI: 10.3791/52139-v • 10:31 min • January 20th, 2015
January 20th, 2015 •
1, 1, 1, 2, 2, 2, 2, 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University, 2Department of Biomedical Physiology and Kinesiology, Simon Fraser University

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Dieses Protokoll zeigt, wie Proximity Ligation Assay kann verwendet werden, um in situ Protein-Protein-Wechselwirkungen an der Drosophila Larvenneuromuskulären Verbindung nachzuweisen. Mit dieser Technik werden Discs großen und Hu-li tai Shao gezeigt, um einen Komplex an der postsynaptischen Region, eine Vereinigung die vorher durch Co-Immunpräzipitation identifiziert bilden.

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Neuroscience Ausgabe 95 adducin Körperwand Dissektion Entwicklungsbiologie Discs großen, Immunhistochemie neuromuskulären Synapse Neurowissenschaften der Protein-Protein-Interaktion Proximity Ligation Assay dritten Larvenstadium
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