An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Starlyn L. M. Okada; Nicole S. Stivers; Peter K. Stys; David P. Stirling

doi:10.3791/52173

Journal

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신경과학

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전의 VIVO 레이저에 의한 척수 손상 모델은 실시간으로 축삭 변성의 메커니즘을 평가하기 위해

An <em>Ex Vivo</em> Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

JoVE 신문
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JoVE 신문 신경과학

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

전의 VIVO 레이저에 의한 척수 손상 모델은 실시간으로 축삭 변성의 메커니즘을 평가하기 위해

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10,854 Views

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11:18 min

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November 25, 2014

DOI:

10.3791/52173-v

DOI:

10.3791/52173-v

•

11:18 min

November 25, 2014

•

10839 Views

Starlyn L. M. Okada¹, Nicole S. Stivers¹, Peter K. Stys², David P. Stirling¹

¹KY Spinal Cord Injury Research Center, Department of Neurological Surgery,University of Louisville ²Hotchkiss Brain Institute, Department of Clinical Neurosciences,University of Calgary

Summary

We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.