Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

Mahdieh Tabatabaei Shafiei; Catalina M. Carvajal Gonczi; Mohammed Samiur Rahman; Ashley East; Jonathan Fran&#231;ois; Peter J. Darlington

doi:10.3791/52199

Journal

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면역학 및 감염병학

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요오드 산 쉬프 염색 말초 혈액 단핵 세포에서 글리코겐을 감지

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

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면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

요오드 산 쉬프 염색 말초 혈액 단핵 세포에서 글리코겐을 감지

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21,112 Views

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09:42 min

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December 23, 2014

DOI:

10.3791/52199-v

DOI:

10.3791/52199-v

•

09:42 min

December 23, 2014

•

10 Views

Mahdieh Tabatabaei Shafiei¹, Catalina M. Carvajal Gonczi¹, Mohammed Samiur Rahman², Ashley East³, Jonathan François³, Peter J. Darlington^1,3

¹Department of Biology,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, ²Department of Chemistry and Biochemistry,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, ³Department of Exercise Science,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University

Summary

Periodic acid Schiff staining is a technique that visualizes the polysaccharide content of tissues. This article demonstrates periodic acid Schiff staining protocol adapted for use on peripheral blood mononuclear cells purified from human venous blood. Such samples are enriched for lymphocytes and other white blood cells of the immune system.