<em>In Vivo</em> and <em>Ex Vivo</em> Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose

Venkatesh Krishnan; Robert Clark; Marina Chekmareva; Amy Johnson; Sophia George; Patricia Shaw; Victoria Seewaldt; Carrie Rinker-Schaeffer

doi:10.3791/52721

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생체 내 및 생체 외 복막 지방이에 밀키 스팟 구조의 난소 암 전이성 식민지를 연구하는 접근 방식

<em>In Vivo</em> and <em>Ex Vivo</em> Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose

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In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose

생체 내 및 생체 외 복막 지방이에 밀키 스팟 구조의 난소 암 전이성 식민지를 연구하는 접근 방식

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13,097 Views

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13:04 min

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October 14, 2015

DOI:

10.3791/52721-v

DOI:

10.3791/52721-v

•

13:04 min

October 14, 2015

•

13040 Views

Venkatesh Krishnan¹, Robert Clark¹, Marina Chekmareva², Amy Johnson¹, Sophia George³, Patricia Shaw⁴, Victoria Seewaldt^4,5, Carrie Rinker-Schaeffer¹

¹Section of Urology, Department of Surgery,The University of Chicago ²Department of Pathology,Robert Wood Johnson Medical School ³Campbell Family Institute for Breast Cancer Research, Princess Margaret Cancer Centre,University Health Network ⁴Department of Laboratory Medicine and Pathobiology, Campbell Family Institute for Breast Cancer Research, Princess Margaret Cancer Centre,University of Toronto, University Health Network ⁵Departments of Medicine, Pharmacology, and Cancer Biology,Duke University Medical Center

Summary

We outline a protocol that implements both in vivo and ex vivo approaches to study ovarian cancer colonization of peritoneal adipose tissues, particularly the omentum. Furthermore, we present a protocol to quantitate and analyze immune cell-structures in the omentum known as milky spots, which promote metastases of peritoneal adipose.