Synthesis of Protein Bioconjugates <em>via</em> Cysteine-maleimide Chemistry

Alexander F. Mason; Pall Thordarson

doi:10.3791/54157

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Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

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Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

38,096 Views

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09:14 min

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July 20, 2016

DOI:

10.3791/54157-v

DOI:

10.3791/54157-v

•

09:14 min

July 20, 2016

•

46 Views

Alexander F. Mason¹, Pall Thordarson¹

¹School of Chemistry, The Australian Centre for Nanomedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,The University of New South Wales

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Summary

This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.

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Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

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