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Journal / Genetics / Ultralow inngang genomet sekvensering biblioteket …
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Genetics
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Ultralow inngang genomet sekvensering biblioteket forberedelse fra en enkelt Tardigrade prøven
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Ultralow inngang genomet sekvensering biblioteket forberedelse fra en enkelt Tardigrade prøven

Article DOI: 10.3791/57615-v • 10:28 min • July 15th, 2018
July 15th, 2018 •
1,2, 1,3, 1,3, 1,3, 1,2,3, 1,2,3
1Institute for Advanced Biosciences, Keio University, 2Systems Biology Program, Graduate School of Media and Governance, Keio University, 3Faculty of Environment and Information Studies, Keio University

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Forurensing under den genomisk sekvensering av mikroskopiske organismer er fortsatt et stort problem. Her viser vi en metode for å sekvens genomet av en Bjørnedyr fra en enkelt prøve med 50 pg genomisk DNA uten hele genomet forsterkning å minimere risikoen for forurensning.

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Genetikk problemet 137 Ultralow inngang forurensning-fri genomet sekvensering Bjørnedyr Hypsibius dujardini fryse-Tin homogenisering
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