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Biochemistry

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JoVE Journal Biochemistry
En gjær 2-Hybrid skjerm i Batch sammenligne Protein interaksjoner
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
Journal (Biochemistry)
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
Journal (Biochemistry)
Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
 
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En gjær 2-Hybrid skjerm i Batch sammenligne Protein interaksjoner

Article DOI: 10.3791/57801-v • 14:23 min • June 6th, 2018
June 6th, 2018 •
1, 1, 1
1Molecular Physiology and Biophysics, University of Iowa

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Satsvis behandling av gjær 2-hybrid-skjermer tillater direkte sammenligning av samhandling profiler av flere agn proteiner med en svært komplekse byttedyr fusion proteiner. Her beskriver vi raffinert metoder, nye reagenser og hvordan du implementerer bruken for slike skjermer.

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Biokjemi problemet 136 Protein interaksjon neste generasjons sekvensering DNA sekvens analyse gjær 2-Hybrid
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