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Journal / Cancer Research / Jäst som chassi för att utveckla funktionella…
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Cancer Research
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JoVE Journal Cancer Research
Jäst som chassi för att utveckla funktionella analyser för att studera humant p53
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Jäst som chassi för att utveckla funktionella analyser för att studera humant p53

Article DOI: 10.3791/59071-v • 14:57 min • August 4th, 2019
August 4th, 2019 •
1, 2, 3, *3, *1, *2
1Mutagenesis and Cancer Prevention Unit, Ospedale Policlinico San Martino, 2Department CIBIO, University of Trento, 3LAQV/REQUIMTE, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto
* These authors contributed equally

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Presenteras här är fyra protokoll för att konstruera och exploatera jäst Saccharomyces cerevisiae reporter stammar att studera humant p53 transaktiveringspotential, effekterna av dess olika cancerrelaterade mutationer, Co-uttryckta samverkande proteiner, och effekterna av specifika små molekyler.

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Cancer forskning p53 jäst Response element funktionell analys transkription tillväxthämning
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