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En laser Capture Micro Dissection protokol, der giver høj kvalitet RNA fra fersk-frosne mus knogler

Article DOI: 10.3791/60197-v • 10:11 min • September 16th, 2019
September 16th, 2019 •
1, 1, 1, 1, 1
1Department of Biomedical Research, University of Veterinary Medicine Vienna

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En laser Capture microdissection (LCM) protokol blev udviklet for at opnå tilstrækkelig mængde af høj kvalitet RNA til genekspression analyse i knogleceller. Den aktuelle undersøgelse fokuserer på musens femur sektioner. Den LCM-protokol, der rapporteres her, kan dog bruges til at studere genekspression i celler i ethvert hårdt væv.

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Biologi laser opsamling microdissection mus knogler RNA genekspression RNA integritets nummer
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