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Genetisk modifikation af cyanobakterier ved konjugering ved hjælp af det modulære Cyanogat klonings værktøjssæt
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Genetisk modifikation af cyanobakterier ved konjugering ved hjælp af det modulære Cyanogat klonings værktøjssæt

Article DOI: 10.3791/60451-v • 08:25 min • October 31st, 2019
October 31st, 2019 •
*1,2,3, *1,2, *1,2, 2,3, 1,2
1Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, 2Centre for Synthetic and Systems Biology, University of Edinburgh, 3Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh
* These authors contributed equally

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Her præsenterer vi en protokol, der beskriver hvordan i) samler en selvkopierende vektor ved hjælp af den modulære CyanoGate klonings værktøjssæt, II) indfører vektoren i en cyanobakterial vært ved konjugering, og III) karakteriserer transgene cyanobakterier stammer ved hjælp af en plade læseren eller flowcytometri.

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Biologi flow cytometry fluorescens Golden Gate plade læser Synechocystis Sp. pcc 6803 Synechococcus elongatus utex 2973 Toolkit forbigående udtryk
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