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Journal / Chemistry / Ein Femtoliter Droplet Array für massive…
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JoVE Journal Chemistry
Ein Femtoliter Droplet Array für massive parallele Proteinsynthese aus einzelnen DNA-Molekülen
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Ein Femtoliter Droplet Array für massive parallele Proteinsynthese aus einzelnen DNA-Molekülen

Article DOI: 10.3791/60945-v • 10:45 min • June 20th, 2020
June 20th, 2020 •
1, 1, 2, 1, 3
1SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2Center for Mathematical Science and Advanced Technology, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Deep-Sea Nanoscience Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

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Das übergeordnete Ziel des Protokolls ist es, über eine Million geordnete, einheitliche, stabile und biokompatible Femtolitertröpfchen auf einem 1 cm2 planaren Substrat herzustellen, das für die zellfreie Proteinsynthese verwendet werden kann.

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Chemie Ausgabe 160 Mikrofabrikation Tröpfchen Femtoliter zellfreie Proteinsynthese fluoreszierendes Protein Enzym Einzelmolekül Poissonverteilung Bildanalyse In-vitro-Kompartimentisierung
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