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Developmental Biology

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Konfokal mikroskop-baserede laser ablation og regenerering assay i zebrafisk interneuromast celler

Article DOI: 10.3791/60966-v • 11:17 min • May 20th, 2020
May 20th, 2020 •
1, 1, 1
1Department of Biology, Pace University

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Laser ablation er en bredt anvendelig teknologi til at studere regenerering i biologiske systemer. Den præsenterede protokol beskriver brugen af et standard laserscanningskonfokalt mikroskop til laserablation og efterfølgende time-lapse-billeddannelse af regenererende interneuromastceller i zebrafisk laterallinjen.

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Udviklingsmæssige Biologi lateral linje interneuromast neuromast hårcelle kappe celle støtte celle ET20 regenerering laser ablation konfokale 405 nm
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