Over the past few decades, changing environmental conditions have been one of the predominant factors that have impacted the quality of life on earth for animals, including humans. For instance, growing evidence on climate change has predicted a rise in the frequency and severity of drought in response to high temperature-humidity imbalance on a global scale. Changing environments also result from the occurrence of various stressful conditions such as extreme pH and oxygen, salinity, and toxicants/ contaminants such as heavy metals, micro- and nano-plastics, etc. Under the global sustainable development agendas, research priorities on “climate action” and “life on land” have warranted attention. In this context, among small animals, insects have proved to be reliable and tractable biological systems to strengthen our understanding of the cause-and-effect relations of environmental change.

The goal of this collection is to curate standardized methodologies for the qualitative and quantitative detection of environmental stress response using insects as representative animal models. This compilation combines the advantage of a traditional manuscript that provides foundational basis to the enquiry with video-based demonstrations to aid reproducibility, accuracy, and accessibility. We invite submissions that advance our understanding of the impact of extreme environmental conditions from behavioral, physiological, biochemical, neurobiological, hormonal, and molecular perspectives under the umbrella of insect stress biology research. Contributions on in vitro and in vivo studies as well as field- and laboratory- based investigations, microscopy techniques, biophysical and biochemical methods, chemical and genetic manipulations, and validation tools based on robust statistical and computational analyses are welcome.