Advancements in ultrasound transducer and computer technology have permitted for the visualization of cardiac structures with excellent spatial and temporal resolution in three dimensions. 3D echocardiography provided new insights into, among others, understanding cardiac mechanics and valvular heart diseases.. From a fancy research tool, 3D echocardiography evolved into a robust clinical modality and several 3D-derived measurements are now incorporated in current clinical guidelines. However, the widespread use of the technique is hindered by its learning curve. The current JoVE collection brings together the cutting-edge protocols of 3D image acquisition and the subsequent analysis to facilitate the use of 3D echocardiography in research and in clinical practice. Invited contributors are experts and everyday users of the technique and will cover the important fields of the assessment of left ventricular, left atrial, and right ventricular morphology.