Methods Collections

Comprehensive Guide to Inflammation Detection Methodologies

Collection Overview

Inflammation is the natural response of the body to harmful stimuli and inflammatory symptoms are routinely used to diagnose ailments. However, acute and chronic inflammation display differing characteristics, which requires deeper exploration for accurate identification. The tools that are currently available are often lacking in this aspect. With the rapid advancement in the field of immunotherapies, there has been a surge in interest in developing effective methodologies to gain a deeper insi Show More



Standardization of splenectomy in a mouse model: A step by step procedure

Shengwen Calvin Li*1
1CHOC Children's Research Institute, UC Irvine

The spleen produces protective humoral antibodies via plasma cells, harbors B and T cell populations, filters particulate matter such as bacteria and antigens, and serves as reservoir for red and white blood cells. The spleen has specific subsets of T cells that may play a role in tumor surveillance and definitively has specific populations of B cells that respond to recognition of encapsulated gram-negative bacteria. Asplenia can impact physiology and promote pathology such as postsplenectomy s Show More

Quantitative imaging of lung homing human lymphocytes in a humanized mouse model of allergic inflammation based on light-sheet fluorescence microscopy.

Anja Schulz-Kuhnt*1, Sebastian Zundler1, Anika Klingberg2, Clemens Neufert1, Stefan Wirtz1, Markus Neurath1, Imke Atreya1
1Department of Medicine 1, University Hospital of Erlangen, Germany, 2Department of Medicine 3, University Hospital of Erlangen, Germany

Overwhelming tissue accumulation of highly activated immune cells represents a hallmark of various chronic inflammatory diseases and emerged as an attractive therapeutic target in the clinical management of affected patients. In order to further optimize strategies aiming on a therapeutic regulation of pathologically imbalanced tissue infiltration of pro-inflammatory immune cells, it will be of particular importance to achieve improved insights into disease- and organ-specific homing properties Show More

Separation of rat epidermis and dermis with thermolysin to detect site-specific inflammatory mRNA and protein.

Kenneth Miller*1
1Oklahoma State University Center for Health Sciences

Thermolysin separation of the rat epidermis and dermis from hindpaw glabrous skin was used to evaluate inflammatory and neurotrophic RNA and proteins using two inflammatory models, subcutaneous injection of: 1) carrageen; 2) complete Freund’s adjuvant. After 6 and 12 hours of inflammation, adult Sprague Dawley rats were euthanized and the hindpaw skin was removed. Skin was incubated in thermolysin (0.5mg/ml) for 2.0-2.5hr in PBS with 1mM calcium chloride at 4°C. The dermis was secured Show More

Assessment of oxidative damage in the primary mouse ocular surface stem cells, in response to, UVC damage

Bipasha Bose*1, Saketh Kapoor1, Utsav Sen1, Sudheer Shenoy P*1
1Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University)

Ocular surface is subjected to regular wear and tear due to various environmental factors. The ocular surfaces of the lit operators or workers such as welders are exposed to wavelengths corresponding to UVC. Here, we demonstrate the exposure of primary cells from the mouse ocular surface to UVC. Reactive oxygen species (ROS) formation is the readout of the extent of oxidative stress/damage. In an experimental in-vitro setting, it is also essential to assess the percentage of dead cells generated Show More

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