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Methods Collections

Established Methods and Innovative Approaches to Fertility Preservation in Mammals

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Methods Collections
Established Methods and Innovative Approaches to Fertility Preservation in Mammals

Guest Editors
Federica Franciosi

University of Milan

Dr. Franciosi is an Assistant Professor of Veterinary Anatomy and Physiology at the University of Milan since 2017 and is …

Collection Overview

Prolonged lifespan and delayed childbearing have prompted the research of reliable methods to preserve fertility for later in life. Such interventions that heavily rely on the cryopreservation of mature gametes (eggs or sperm) and embryos generally reach a satisfactory success rate. However, they are not applicable when mature gametes are not available (for instance in pediatric oncologic patients). Developing effective methods to prevent the loss of the reproductive function (or to restore it) is an emerging need for the increasing population of cancer survivors, as well as for patients with other types of gonadal insufficiency.

Besides human reproductive medicine, the development of fertility preservation techniques have found broad applications in animal husbandry and for the protection of endangered species; this is through the salvage and propagation of precious genetic resources. Finally, the scientific community greatly benefits from these new methods for fertility preservation through sharing complex transgenic animal models worldwide and the cost-effective maintenance of transgenic animal colonies.

The goal of this Methods Collection is two-fold: (1) to provide detailed state-of-the-art protocols and procedures currently in use in clinical and other ‘field’ settings; and (2) to introduce novel approaches, initially developed as research models, that have the potential to translate into field applications. Ultimately, we believe that this in-depth sharing of knowledge and techniques will improve the outcome and the safety of fertility preservation interventions and expand the array of currently available technologies.





Laparoscopic Oocyte Retrieval and Oocyte Cryopreservation during Vaginoplasty to Reduce Invasiveness in the Treatment of Mayer-Rokitansky-Kuster-Hauser (MRKH) Syndrome

Valeria Stella Vanni1, 2, Alessandra Alteri*1, 2, Greta Chiara Cermisoni1, Lucia De Santis1, 2, Elisa Rabellotti1, Diana Del Prato1, Marta Parma1, Enrico Papaleo1, Luigi Fedele3, Massimo Candiani1, 2
1Obstetrics and Gynaecology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy, 2Vita-Salute San Raffaele University, Milan, Italy, 3Department of Obstetrics and Gynecology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milan, Italy

CO2 Fiber Laser Vaporization of Ovarian Endometrioma: an Ovarian Sparing Procedure.

Massimo Candiani*1, Jessica Ottolina1, Iacopo Tandoi1, Ludovica Bartiromo1, Matteo Schimberni1, Stefano Ferrari1
1Gynaecological Surgery and Endometriosis Departmental Unit, San Raffaele Scientific Institute, Milan, Italy

Fertility preservation in patients with severe ovarian dysfunction

Kazuhiro Kawamura*1, Yuta Kawagoe1
1International University of Health and Welfare School of Medicine

Modelling fertility potential, an introduction of modern statistical and computational methods

Yan Yuan*1
1University of Alberta

Vitrification of adult and juvenile sheep oocytes: challenges and opportunities

Fiammetta Berlinguer*1, Giovanni G. Leoni1, Salvatore Naitana1, Sara Succu1
1University of Sassari - Department of Veterinary Medicine

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