Methods for studying CRISPR-Cas systems
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Institute of Microbiology
I’m a postdoctoral researcher in Agata Krawczyk-Balska’s lab at University of Warsaw. My current research...
Due to their unique mode of action, CRISPR-Cas systems employ multiple sophisticated mechanisms. These include numerous interactions between various types of molecules (e.g., RNA, DNA, and proteins). Precise description of these interactions is crucial both for basic studies as well for designing practical applications for CRISPR-Cas systems of various types. Yet, it is not an easy task to adapt existing methods to achieve reliable results in this uneasy field. The aim of this collection is to gather methods developed and optimized for studying CRISPR-Cas systems at each stage of their activity: spacer acquisition, biogenesis, and interference.
1Department of Neuroscience, University of Arizona, 2School of Life Sciences, Arizona State University, 3Department of Scientific and Technologic Investigations, University of Sonora, 4Integrated DNA Technologies, Inc.
1DSM Biotechnology Center, 2Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, 3BrisSynBio, University of Bristol, Life Sciences Building, 4School of Biological Sciences, University of Bristol, Life Sciences Building