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Methods Collections

RNA/DNA In Situ Hybridization techniques

Collection Overview

In Situ Hybridization (ISH) provides a powerful tool for localized detection of RNA/DNA sequences, widely applied in both research and clinical contexts. This technique takes advantage of nucleic acid probes that are complementary to target RNA or DNA sequences, in order to localize the target sequences on chromosomes, in tissues, cells, organs or organisms. RNA ISH can be used to identify deregulated expression of mRNA, lncRNA, snRNA or miRNA transcripts, which can manifest as expression mis-lo Show More


Next generation RNAscope/DNAscope in situ hybridization applications in HIV/SIV research

Catherine Brands1, David Morcock1, Jacob D. Estes2, Claire Deleage*1
1Frederick National Laboratory for Cancer Research, 2Vaccine and Gene Therapy Institute, Oregon National Primate Research Center

In situ hybridization is a powerful technique to identify specific RNA or DNA sequences within individual cells in tissue sections, providing important insights into physiological processes and disease pathogenesis. ISH has been used for many years to assess the location of cells infected by viruses, but recently a next-generation ISH approach (termed RNAscope) was developed with a unique probe design strategy that allows simultaneous signal amplification and background suppression to achieve si Show More

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