Laboratory of Pharmacology and Pharmaceutical Care, Faculty of Pharmacy, University of Coimbra
The vascular system exerts key functions in the human body, particularly by regulating cardiovascular homeostasis. Accordingly, vascular dysfunction has been linked to atherogenesis and cardiovascular disease. Since the discovery of the vascular endothelium and its crucial role in cardiovascular physiology, tremendous advances have been observed in this field of research. Furthermore, membrane receptors such as G-protein coupled receptors have been implicated in a wide array of physiological mechanisms, including in the vascular system.
The present Methods Collection aims at providing a comprehensive guide of the main methods currently available to probe the localization, expression, and function of membrane receptors in the vascular system. Examples of methods to be included are proteomic and genomic assays, radioligand binding assays, autoradiography, immunolabeling and methods for vascular response assessment, including isolated organ and cascade superfusion techniques.