Methods in Allergy
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Universidad de Málaga
Dr. María Jose holds an MD and a PhD degree in medicine and surgery from the University of Malaga. She is an…
Instituto de Investigación Sanitaria de Alicante-ISABIAL-Miguel Hernández University
Dr. Javier holds an MD and a PhD degree in Medicine and Surgery from the University of Valladolid, in Spain. He is an…
There are two kinds of methods in allergy, namely in vivo and in vitro. Our proposal consists of three in in vivo methods and two in vitro methods.The in vivo methods consist of skin tests, patch tests, and nasal provocation tests.Skin tests include prick and intradermal tests which cover the most practical and useful methods for most of allergic diseases (Rhinitis, asthma, or food allergy). Patch tests cover delay of allergic reactions (contact dermatitis). Nasal provocation test is one of the gold standard in knowing the causal allergens and in the last few years, it is the only valid method to differentiate between local or general allergy rhinitis (endotypes and phenotypes), because in the first type skin tests or IgE in vitro methods are negatives. The in vitro methods consist of basophil activation test and Immunoassay platforms for simultaneous measurement of specific IgE antibodies. Basophil activation test is one of the most promising in vitro method to detect activation of basophils by IgE or direct basophil activation. As currently there are no other methods for IgE detection in vitro. Also, it can be useful in the follow up of venom allergic patients or drug allergy. Immunoassay platforms Simultaneous measurement of specific IgE antibodies are used since the obtention of molecular allergens, there are several platforms to detect specific IgE antibodies to multiple allergens. The most known is ISACTM from Thermofisher S. Inc. But there are more as Euroline™ from EUROIMMUN AG, or Polycheck® from Biocheck GmbH. They are especially useful in case of cross reactivity, that is very common diagnostic problem in allergy.