Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Methods Collections

Advanced Glycomics and Glycoproteomics Methods

COVID-19 response: Video production in affected areas is postponed, publishing text articles first

JoVE will publish text articles after the peer review, which on average takes two months after the manuscript submission. We will film and add corresponding videos to our website when laboratories and facilities in affected areas reopen.

Just Started
Methods Collection Image
Methods Collections
Advanced Glycomics and Glycoproteomics Methods

Guest Editors
Yehia Mechref

Texas Tech University

Dr. Yehia Mechref received his BS in Chemistry at the American University of Beirut, Beirut, Lebanon, and a PhD with an…

Collection Overview

Glycosylation of proteins is the most common modification that modulates the biological functions of vital biomolecules. Glycosylation of proteins involves the addition and trimming of several monosaccharides controlled by actions of numerous transferases and exoglycosidases. The glycosylation process's complexity results in highly diverse glycan structures and unique characteristics of cells, tissues, and organisms. Mass Spectrometry-based glycomics and glycoproteomics continue to be highly dynamic and exciting research areas due to the need to comprehensively understand the biological attributes of protein glycosylation in many critical biological functions. Moreover, aberrant glycosylation has been recognized for decades as the trait of many mammalian diseases, including osteoarthritis, cystic fibrosis, and cancer. Although glycoproteins and glycopeptides have attracted significant interests for years, a comprehensive characterization of protein glycosylation microheterogeneity continues to be an arduous task, complicated by the presence of multiple sites occupied by a large number of isomeric structures. Additionally, isomeric separation of glycans and glycopeptides remains a daunting analytical task. Therefore, the development of analytical methods, sensitive and efficient in characterizing the diverse glycan structures, associated with proteins is still in demand and highly desired.

Analytical methods employing cutting-edge techniques such as mass spectrometry (MS), chromatography, and capillary electrophoresis, have been developed to characterize the glycan moieties of glycoproteins unequivocally. This Method Collection will highlight state-of-the-art methods that have been explicitly designed to characterize glycans, glycoproteins, and glycopeptides derived from biological samples.

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter