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January 11, 2011
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Automatically generated
The ability to produce transgenes for Caenorhabditis elegans using genomic DNA carried by fosmids is particularly attractive as all of the native regulatory elements are retained. Described is a simple and robust procedure for the production of transgenes via recombineering with the galK selectable marker.
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Zhang, Y., Kashyap, L., Ferguson, A. A., Fisher, A. L. The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker. J. Vis. Exp. (47), e2331, doi:10.3791/2331 (2011).
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