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December 12, 2011
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Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
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Mouse Sperm CryopreservationI·Cryo KitGenetically Modified MiceTransgenesisKnockout TechnologiesCryopreservation Of EmbryosCryoprotective AgentRaffinoseSkim MilkArchiving And Distribution Of StrainsRecovery Of Valuable StrainsSperm MotilityProgressive MotilityInbred Mouse StrainsTwo-cell Stage Embryo DevelopmentIVFGM Mouse Lines
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Liu, L., Sansing, S. R., Morse, I. S., Pritchett-Corning, K. R. Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit. J. Vis. Exp. (58), e3713, doi:10.3791/3713 (2011).
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