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11:50 min
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September 10, 2014
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Cancer stem cells (CSCs) are implicated in tumor relapse due to chemoresistance. We have optimized a protocol for selection and expansion of CSCs from ovarian cancer cell lines. By treating cells with the chemotherapeutic cisplatin and culturing in a stem cell promoting media we enrich for non-adherent CSC cultures.
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Cole, J. M., Joseph, S., Sudhahar, C. G., Cowden Dahl, K. D. Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines. J. Vis. Exp. (91), e51891, doi:10.3791/51891 (2014).
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