Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

Chi-Hao Hsiao; Ya-Hsu Chiu; Shao-Chih Chiu; Der-Yang Cho; Liang-Ming Lee; Yu-Ching Wen; Jia-Jhe Li; Ping-Hsiao Shih

doi:10.3791/60420

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Badania Nad Rakiem

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Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

JoVE Journal
Badania Nad Rakiem

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JoVE Journal Badania Nad Rakiem

Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

10,741 Views

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11:11 min

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January 24, 2020

DOI:

10.3791/60420-v

DOI:

10.3791/60420-v

•

11:11 min

January 24, 2020

•

13 Views

Chi-Hao Hsiao^1,2,3, Ya-Hsu Chiu⁴, Shao-Chih Chiu^4,5, Der-Yang Cho^4,6,7, Liang-Ming Lee^1,2, Yu-Ching Wen^1,2, Jia-Jhe Li⁴, Ping-Hsiao Shih⁴

¹Department of Urology,Wan Fang Hospital, ²Department of Urology, School of Medicine, College of Medicine,Taipei Medical University, ³Graduate Institute of Clinical Medicine, College of Medicine,Taipei Medical University, ⁴Translational Cell Therapy Center, Department of Medical Research,China Medical University Hospital, ⁵Graduate Institute of Biomedical Sciences,China Medical University, ⁶Graduate Institute of Immunology,China Medical University, ⁷Department of Neurosurgery, Neuropsychiatric Center,China Medical University Hospital

Transkrypcja

Podsumowanie

Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3⁺CD56⁺ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.