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June 23, 2020
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The presented approach simultaneously evaluates cancer cell invasion in 3D spheroid assays and T-cell cytotoxicity. Spheroids are generated in a scaffold-free agarose multi-microwell cast. Co-culture and embedding in type I collagen matrix are performed within the same device which allows to monitor cancer cell invasion and T-cell mediated cytotoxicity.
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Lin, Y., Nasir, A., Camacho, S., Berry, D. L., Schmidt, M. O., Pearson, G. W., Riegel, A. T., Wellstein, A. Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture. J. Vis. Exp. (160), e61392, doi:10.3791/61392 (2020).
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