Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

Limei Wang; Jianmin Guo; Xiaoman Zhong; Jian Zhang; Yali Sheng; Qiwen Lai; Hui Song; Wei Yang

doi:10.3791/63439

Powtarzające się pobieranie próbek krwi z żyły podobojczykowej przytomnego szczura

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JoVE Journal Medicine

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

Powtarzające się pobieranie próbek krwi z żyły podobojczykowej przytomnego szczura

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04:52 min

February 9, 2022

DOI: 10.3791/63439-v

Limei Wang¹, Jianmin Guo¹, Xiaoman Zhong¹, Jian Zhang², Yali Sheng¹, Qiwen Lai¹, Hui Song¹, Wei Yang¹

¹Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research, TCM Non-clinic Evaluation Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine,Guangdong Engineering Research Center for Innovative Drug Evaluation and Research, ²Department of Biomedical Engineering, School of Basic Medical Sciences,the Sixth Affiliated Hospital of Guangzhou Medical University

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Overview

This protocol describes a simple and efficient method for collecting blood from the subclavian vein in rats. It allows for rapid and easily identifiable sampling without anesthesia, ensuring high-quality blood collection through repetitive sampling.

Key Study Components

Area of Science

Pharmacokinetics
Toxicokinetics
Animal Research

Background

Blood collection is essential for studying drug exposure in rats.
The subclavian vein is a suitable site for blood sampling.
Understanding rat anatomy is crucial for successful sampling.
Qualified professionals should perform this method due to its risks.

Purpose of Study

To provide a reliable method for blood collection in pharmacokinetic studies.
To enable repeat sampling without the need for anesthesia.
To ensure high-quality blood samples for analysis.

Methods Used

Locate the subclavian triangular fossa in the rat.
Use appropriate restraint techniques for the rat.
Employ a qualified professional for the procedure.
Collect blood samples at specific time points.

Main Results

The method allows for timely and identifiable blood sampling.
High-quality blood is obtained through repetitive collection.
The technique is effective for pharmacokinetic and toxicokinetic studies.
Risks associated with the method require professional oversight.

Conclusions

This protocol provides an efficient approach to blood collection in rats.
It supports the needs of pharmacokinetic research.
Proper training and knowledge of anatomy are essential for success.

Frequently Asked Questions

What is the main advantage of this blood collection method?

The main advantage is that it allows for repeat sampling without anesthesia, ensuring high-quality blood collection.

Is this method safe for the rats?

While the method is effective, it carries risks and should only be performed by qualified professionals.

What anatomical knowledge is required for this procedure?

Understanding the anatomy of the subclavian vein and proper restraint techniques is crucial for successful sampling.

Can this method be used for other types of studies?

Yes, it is primarily used for pharmacokinetics and toxicokinetics but may be applicable in other research areas requiring blood sampling.

How does this method compare to other blood collection techniques?

This method is simpler and allows for repeated sampling without anesthesia, which can be advantageous in certain studies.

What precautions should be taken during the procedure?

Precautions include ensuring the procedure is performed by trained personnel and monitoring the rat for any signs of distress.

Obecny protokół opisuje prostą i efektywną metodę pobierania krwi z żyły podobojczykowej u szczurów. Umożliwia szybkie, terminowe i łatwe do zidentyfikowania pobieranie próbek bez znieczulenia oraz pozwala uzyskać wysokiej jakości krew dzięki powtarzalnemu pobieraniu próbek.

W badaniach farmakokinetycznych i toksykokinetycznych musimy pobrać pewną ilość krwi w określonych punktach czasowych u szczurów, aby wykryć ekspozycję na lek. W badaniu tym opisano prostą i skuteczną metodę pobierania krwi z żyły podobojczykowej u szczurów w tym protokole. Technika ta umożliwia powtarzalne, terminowe i łatwe do zidentyfikowania pobieranie próbek bez znieczulenia oraz uzyskanie wysokiej jakości krwi poprzez powtarzalne pobieranie próbek.

Powinieneś znać anatomię żyły podobojczykowej, zdolne do unieruchomienia szczurów i czubek igły. Metoda jest ryzykowna i wymaga wykwalifikowanego specjalisty. Na początek znajdź najniższy punkt trójkątnego dołu podobojczykowego między szyją a kończyną przednią u szczura.

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