Jove Lab Chem
Temperature Dependence — Lab Prep
Source: Smaa Koraym at Johns Hopkins University, MD, USA
- Preparation of 0.5 M Fe(NO3)3
Here, we show the preparation of a solution for 10 students working in pairs, with some excess. Please adjust quantities as needed.
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To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves
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Prepare 250 mL of a 0.5 M aqueous solution of iron (III) nitrate. Measure out 50.5 g of iron nitrate nonahydrate on a top-loading balance. Place a powder funnel in the mouth of a 250-mL Erlenmeyer flask and pour the iron (III) nitrate nonahydrate into it.
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Measure out 175 mL of deionized water with a graduated cylinder and pour it into the flask.
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Remove the funnel, place a stir bar in the flask, and start stirring the mixture on a stir plate. While the mixture stirs, label a 250-mL polyethylene bottle as ‘0.5 M Fe(NO3)3’.
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Once the solution appears homogeneous, place the funnel back in the flask and add another 75 mL of deionized water.
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Continue stirring until the solution appears homogeneous again. Then, turn off the stir motor and remove the stir bar from the flask.
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Transfer the solution into the polyethylene bottle. Store the capped bottle at the back of the instructor's hood until just before the lab.
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Lastly, wash your glassware and equipment and put away the iron (III) nitrate nonahydrate.
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- Preparation of the Laboratory
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Place a labeled waste container for aqueous iron solutions in the back of the hood.
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Make sure each workstation is equipped with a hotplate and a support rod, a medium 3-prong clamp, and a thermometer clamp. Attach both clamps to the support rod, and confirm that the vacuum sources are equipped with silicone tubing.
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Ensure that each sink has plenty of paper towels.
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Assemble the gas pressure sensor devices. Confirm that the devices are charged and working before distributing them to the workstations.
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Make sure two locking tapered stopper adapters are tightly attached to the flexible polymer tubing and set one at each workstation along with a 20-mL Luer-Lok syringe, a two-way locking stopcock, and a number five 2-hole rubber stopper.
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Set out the following equipment and glassware at each lab station (we suggest that students work in pairs):
1 20-mL volumetric pipette 1 Pipette controller 1 50-mL beaker 1 100-mL beaker 3 400-mL beakers 1 600-mL beaker 1 125-mL Büchner flask 1 Roll of lab tape 1 Labeling pen 1 Glass thermometer -
Fill a 125-mL wash bottle with deionized water, and set one at each workstation.
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Shortly before the lab, fill an insulated cooler or bucket with crushed or chipped ice. Place the cooler in a central area.
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Finally, place a 1-L bottle of 3 wt% hydrogen peroxide in the central hood.
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