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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein (Video) | JoVE

Production and Imaging of Recombinant Protein-Filled Vesicles from E. coli

Article DOI: 10.3791/200248-v • 02:50 min • June 30th, 2023
June 30th, 2023 •
1, 1, 1, 1
1School of Biosciences, University of Kent

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Optimized Production Analysis Recombinant Protein Vesicles E. Coli Dynamic Interactions Biochemical Analysis Large Quantities Correctly-folded Functional Form Misfolded Insoluble Bacteria Expression Mammalian Cells Synthetic Polypeptides High Yield Expression Export Of Challenging Proteins Cleavable Peptide Sequence Production And Yield Enhancement Protein Export Media Exportation Downstream Protein Purification Short Peptide Tag Membrane Bound Vesicles Effective Solution Bacterial Recombinant Protein Expression
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