An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Starlyn L. M. Okada; Nicole S. Stivers; Peter K. Stys; David P. Stirling

doi:10.3791/52173

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Um Ex Vivo Induzida por laser Lesão Medular modelo para avaliar mecanismos de axonal Degeneration em tempo real

An <em>Ex Vivo</em> Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

JoVE Journal
Neurociência

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JoVE Journal Neurociência

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Um Ex Vivo Induzida por laser Lesão Medular modelo para avaliar mecanismos de axonal Degeneration em tempo real

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10,862 Views

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11:18 min

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November 25, 2014

DOI:

10.3791/52173-v

DOI:

10.3791/52173-v

•

11:18 min

November 25, 2014

•

10839 Views

Starlyn L. M. Okada¹, Nicole S. Stivers¹, Peter K. Stys², David P. Stirling¹

¹KY Spinal Cord Injury Research Center, Department of Neurological Surgery,University of Louisville ²Hotchkiss Brain Institute, Department of Clinical Neurosciences,University of Calgary

Summary

We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.