Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Kateena Addae Konadu; Ming Bo Huang; William Roth; Wendy Armstrong; Michael Powell; Francois Villinger; Vincent Bond

doi:10.3791/53495

Journal

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Imunologia e Infecção

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Isolamento de exossomas a partir do plasma de indivíduos HIV-1 positivos

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Isolamento de exossomas a partir do plasma de indivíduos HIV-1 positivos

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17,241 Views

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06:46 min

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January 05, 2016

DOI:

10.3791/53495-v

DOI:

10.3791/53495-v

•

06:46 min

January 05, 2016

•

4 Views

Kateena Addae Konadu¹, Ming Bo Huang¹, William Roth¹, Wendy Armstrong², Michael Powell¹, Francois Villinger^3,4, Vincent Bond¹

¹Department of Microbiology, Biochemistry, Immunology,Morehouse School of Medicine, ²Department of Medicine,Emory University School of Medicine, ³Department of Pathology and Laboratory Medicine,Emory University School of Medicine, ⁴Yerkes National Primate Research Center

Transcrição

Summary

Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.