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JoVE Journal Genetics
Voorbijgaande expressie van buitenlandse genen in Insect cellen (sf9) voor eiwit functionele Assay
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Voorbijgaande expressie van buitenlandse genen in Insect cellen (sf9) voor eiwit functionele Assay

Article DOI: 10.3791/56693-v • 11:12 min • February 22nd, 2018
February 22nd, 2018 •
1, 2, 2, 3, 1
1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University

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Dit protocol beschrijft een warmte schok-geïnduceerde proteïne expressie systeem (pDHsp/V5-zijne/sf9 cel), die kan worden gebruikt voor het uitdrukken van vreemde proteïnen of evaluatie van de anti-apoptotic activiteit van potentiële buitenlandse eiwitten en hun afgeknotte amino zuren in insect cellen.

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Genetica kwestie 132 voorbijgaande expressie eiwit functionele assay sf9 cel lijn Lymantria xylina meerdere nucleopolyhedrovirus Ly-inhinbitor van Apoptosis-3 Drosophila warmte schok 70 promotor pDHsp/V5-Hsi Drosophilia Reaper actinomycin-D
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