Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Ana C. Silva; Maria J. Oliveira; Todd C McDevitt; M&#225;rio A. Barbosa; Diana S. Nascimento; Perp&#233;tua Pinto-do-&#211;

doi:10.3791/56924

Decellularization comparável de explantes de tecido cardíaco Fetal e adulto como plataformas 3D-c...

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Bioengineering

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JoVE Journal Bioengineering

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Decellularization comparável de explantes de tecido cardíaco Fetal e adulto como plataformas 3D-como para estudos de Vitro

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08:10 min

March 21, 2019

DOI: 10.3791/56924-v

Ana C. Silva^1,2,3,4, Maria J. Oliveira^1,2,5, Todd C McDevitt^4,6, Mário A. Barbosa^1,2,3, Diana S. Nascimento^1,2, Perpétua Pinto-do-Ó^1,2,3

¹i3S - Instituto de Investigação e Inovação em Saúde,Universidade do Porto, ²INEB - Instituto de Engenharia Biomédica,Universidade do Porto, ³Instituto de Ciências Biomédicas Abel Salazar (ICBAS),Universidade do Porto, ⁴Gladstone Institute of Cardiovascular Disease, ⁵Faculty of Medicine,University of Porto, ⁶University of California San Francisco

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Overview

The cardiac extracellular matrix (ECM) plays a crucial role in the heart's physiological remodeling and function. This study presents a standardized decellularization method for fetal and adult hearts, enabling comparative analysis of their ECM properties in a 3D context.

Key Study Components

Area of Science

Cardiovascular biology
Extracellular matrix research
Comparative tissue analysis

Background

The ECM is essential for tissue structure and function.
Understanding ECM differences between fetal and adult hearts is vital for cardiovascular research.
Decellularization techniques allow for the preservation of native tissue architecture.
This method has been applied successfully to other organs.

Purpose of Study

To investigate the impact of ECM during different developmental stages of the heart.
To facilitate comparative analysis of fetal and adult heart ECM.
To enhance understanding of ECM roles in cardiovascular health and disease.

Methods Used

Decellularization of fetal and adult mouse hearts using a standardized protocol.
Rinsing tissues with ice-cold PBS before dissection.
Dissecting hearts under a microscope to isolate left ventricles.
Dividing left ventricle free walls into strips for analysis.

Main Results

Successful decellularization preserves ECM architecture.
Comparative analysis reveals differences in ECM properties between fetal and adult hearts.
Method applicable to other organs, enhancing ECM research.
Findings contribute to understanding ECM's role in heart development.

Conclusions

The standardized decellularization method is effective for ECM studies.
Insights gained can inform cardiovascular research and therapies.
Future studies may explore ECM implications in heart disease.

Frequently Asked Questions

What is the extracellular matrix?

The extracellular matrix (ECM) is a network of proteins and molecules that provide structural and biochemical support to surrounding cells.

Why is decellularization important?

Decellularization removes cells from tissues while preserving the ECM, allowing for the study of its properties and functions without cellular interference.

How does this study benefit cardiovascular research?

By comparing fetal and adult ECM, researchers can better understand developmental differences and their implications for heart health.

Can this method be applied to other organs?

Yes, the decellularization method has been successfully applied to various organs, facilitating ECM studies in different contexts.

What are the potential applications of this research?

Findings can inform tissue engineering, regenerative medicine, and understanding heart diseases.

A cardíaca matriz extracelular (ECM) é uma rede complexa de moléculas que orquestrar processos-chave em tecidos e órgãos enquanto duradoura remodelamento fisiológico durante toda a vida. Decellularization padronizada do coração fetal e adulta permite estudos comparativos de experimentais de ambos os tecidos em um contexto 3D capturando arquitetura nativa e propriedades biomecânicas.

Este método pode ajudar a abordar questões importantes no campo cardiovascular sobre o impacto que a matriz extracelular tem durante diferentes estágios fisiológicos e de desenvolvimento do coração. A principal vantagem dessa técnica é que permite uma análise comparativa entre matrizes extracelulares fetais e adultas, aplicando um método de descelularização semelhante aos dois tipos de tecido. Assim, esse método tem sido bem sucedido aplicado a outros órgãos, como ressecções cirúrgicas de pacientes com câncer facilitando o estudo da matriz extracelular em diferentes contextos.

Comece enxaguando brevemente os corações de camundongos fetais e adultos com PBS gelado. Em seguida, coloque os tecidos sob um microscópio dissecando e use uma tesoura pequena reta para remover a atria, o ventrículo direito e o septo dos corações do rato adulto. Transfira o ventrículo esquerdo para uma nova placa de Petri e divida a parede livre do ventrículo esquerdo em tiras longitudinais de dois milímetros de espessura.

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