Minimizando a hipóxia em fatias hipocampais de camundongos adultos e envelhecidos
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July 02, 2020
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Este é um protocolo para preparação aguda de fatias de hipocampi de camundongos adultos e envelhecidos que se aproveita da perfusão transcárrica e corte de fatias com NMDG-aCSF gelado para reduzir danos hipóxicos ao tecido. As fatias resultantes permanecem saudáveis durante muitas horas, e são adequadas para grampos de remendo de longo prazo e gravações de campo.
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Djurisic, M. Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice. J. Vis. Exp. (161), e61377, doi:10.3791/61377 (2020).
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