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In vitro Analyse af plasmodiuminficerede røde blodlegemer ved cytotoksiske lymfocytter
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

In vitro Analyse af plasmodiuminficerede røde blodlegemer ved cytotoksiske lymfocytter

DOI:
10.3791/63987-v

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08:20 min

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August 17, 2022

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, , ,
1Instituto René Rachou,Fundação Oswaldo Cruz, 2Departamento de Bioquímica e Imunologia,Universidade Federal de Minas Gerais

Capítulos

  • 00:04Introduction
  • 00:58Experimental Malaria Infection in Mouse
  • 01:49Obtaining Fresh Whole Mouse Splenocytes
  • 03:30Cytotoxic Effector Cell Purification: CD8a+ T Cell Negative Selection
  • 04:22P. yoelii Infected RBC Isolation
  • 05:21CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
  • 06:22Results: P. yoelii – iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii – iRBCs by CD8+ lymphocytes
  • 07:39Conclusion

Summary

Tadução automática

Tadução automática

Her beskriver vi en ny metode til at hjælpe med at belyse mekanismerne for cellulær immunitet over for Plasmodium i blodstadiet af infektion. Dette er et in vitro-assay , der måler inficeret drab af røde blodlegemer ved cytotoksiske lymfocytter.

Tags

In Vitro AssayPlasmodium-infected Red Blood CellsCytotoxic LymphocytesCell-mediated ImmunityMalaria PathogenesisProtective Malaria ImmunitySpleen IsolationRBC LysisMACS Column Purification

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