JOVE JOURNAL

Biochemistry

Showing 13 - 24 in 822 videos.

SPR Analysis to Determine the Binding Affinities of Vitamin A Membrane Receptors with Their Physiological Ligand RBP4

Rakesh Radhakrishnan¹, Anjelynt Lor¹, Dorothy Li¹, Deepti Mudaliar², Glenn P. Lobo¹

¹Department of Ophthalmology and Visual Neurosciences,University of Minnesota, ²High Throughput Screening Laboratory, Institute for Therapeutics Discovery & Development,University of Minnesota

Journal (Biochemistry)

Kinetic Assay Setup for Analyzing the Kinetic Parameters of RBPR2 and STRA6 Interaction with Vitamin A Receptor

Rakesh Radhakrishnan¹, Anjelynt Lor¹, Dorothy Li¹, Deepti Mudaliar², Glenn P. Lobo¹

¹Department of Ophthalmology and Visual Neurosciences,University of Minnesota, ²High Throughput Screening Laboratory, Institute for Therapeutics Discovery & Development,University of Minnesota

Journal (Biochemistry)

Spectrophotometry Methodology to Quantify the GPCR-11-cis Retinal Protein Complex in Retinal Lysates

Rakesh Radhakrishnan¹, Anjelynt Lor¹, Dorothy Li¹, Deepti Mudaliar², Glenn P. Lobo¹

¹Department of Ophthalmology and Visual Neurosciences,University of Minnesota, ²High Throughput Screening Laboratory, Institute for Therapeutics Discovery & Development,University of Minnesota

Journal (Biochemistry)

Methodology for Studying Interactions of Vitamin A Membrane Receptors and Opsin Protein with their Ligands in Generating the Retinylidene Protein

Rakesh Radhakrishnan¹, Anjelynt Lor¹, Dorothy Li¹, Deepti Mudaliar², Glenn P. Lobo¹

¹Department of Ophthalmology and Visual Neurosciences,University of Minnesota, ²High Throughput Screening Laboratory, Institute for Therapeutics Discovery & Development,University of Minnesota

Journal (Biochemistry)

Demonstration of Membrane Transport of Histidine using Goat Intestinal Inverted Sacs: An Experiential Pedagogical Tool for Undergraduates

Hiba Haris¹, Prachi Kumar¹, Vivek Bhardwaj¹, Sidhartha Taritla¹, Vandana Malhotra¹, Nandita Narayanasamy¹

¹Department of Biochemistry, Sri Venkateswara College,University of Delhi

Journal (Biochemistry)

Preparation of Inverted Jejunum Sacs for Demonstrating the Membrane Transport of Histidine

Hiba Haris¹, Prachi Kumar¹, Vivek Bhardwaj¹, Sidhartha Taritla¹, Vandana Malhotra¹, Nandita Narayanasamy¹

¹Department of Biochemistry, Sri Venkateswara College,University of Delhi

Journal (Biochemistry)

Experimental Setup for Histidine Transport and Estimation of Histidine Using Pauly’s Reaction

Hiba Haris¹, Prachi Kumar¹, Vivek Bhardwaj¹, Sidhartha Taritla¹, Vandana Malhotra¹, Nandita Narayanasamy¹

¹Department of Biochemistry, Sri Venkateswara College,University of Delhi

Journal (Biochemistry)

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Cameron McClymont¹, Karolina Chabowska¹, Sherry Xie¹, May Thu Kyaw¹, Hasan Yardimci¹

¹The Francis Crick Institute

Journal (Biochemistry)

Preparation of a Biotin-PEG Flow Cell Assembly for Single Molecule Total Internal Reflection Fluorescence Microscopy

Cameron McClymont¹, Karolina Chabowska¹, Sherry Xie¹, May Thu Kyaw¹, Hasan Yardimci¹

¹The Francis Crick Institute

Journal (Biochemistry)

Single Molecule Total Internal Reflection Fluorescence Assay for Visualizing CMG-Mediated DNA Unwinding

Cameron McClymont¹, Karolina Chabowska¹, Sherry Xie¹, May Thu Kyaw¹, Hasan Yardimci¹

¹The Francis Crick Institute

Journal (Biochemistry)

Preparation of ECF-5 Peptide Hydrogels for Regenerative Medicine Using pH Response Method

Yue Zhao¹, Mai Hu², Yiwen Gong², Yihao Zhao², Zhiwei Shen²

¹The First Affiliated Hospital of Nanchang University, ²The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine,Nanchang University

Journal (Biochemistry)

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels

Yue Zhao¹, Mai Hu², Yiwen Gong², Yihao Zhao², Zhiwei Shen²

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