JOVE JOURNAL

Biochemistry

Showing 37 - 48 in 827 videos.

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

James Sandy¹, Halina Mikolajek^1,2, Amy J. Thompson¹, Juan Sanchez-Weatherby¹, Michael A Hough^1,2

¹Diamond Light Source Ltd,Harwell Science and Innovation Campus, ²Research Complex at Harwell,Harwell Science and Innovation Campus

Journal (Biochemistry)

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Russell Spencer-Smith^1,2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Journal (Biochemistry)

DNAzyme 10-23 – Based Nanomachines for Nucleic Acid Recognition

Pavel V. Filatov¹, Muhannad Ateiah¹, Maria Y. Berezovskaya¹, Maria S. Rubel¹, Dmitry M. Kolpashchikov²

¹ITMO University, ²University of Central Florida

Journal (Biochemistry)

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions

Héctor Cruz^1,2, Alejandro Llanes^2,3, Patricia L. Fernández^2,3

¹Facultad de Ciencias y Tecnología,Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

Journal (Biochemistry)

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Shreenidhi Rajkumar¹, Danielle Dixon¹, Andrew M. Lipchik², Joshua J. Gruber¹

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

Journal (Biochemistry)

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

Myndert Claasen¹, Zornitsa Kofinova¹, Matteo Contino², Weston B. Struwe^3,4

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez¹

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

Journal (Biochemistry)

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

Trinity Cookis¹, Paul Sauer^2,3, Simon Poepsel^4,5, Bong-Gyoon Han⁶, Dominik A. Herbst^1,2,6, Robert Glaeser⁶, Eva Nogales^1,2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Sample Preparation for In Situ Cryotomography of Mammalian Cells

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon

Anjelique Sawh-Gopal^1,2, Aygul Ishemgulova*¹, Eugene Y. D. Chua*¹, Mahira F. Aragon*¹, Joshua H. Mendez¹, Edward T. Eng¹, Alex J. Noble¹

¹Simons Electron Microscopy Center, New York Structural Biology Center, ²Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

Journal (Biochemistry)

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Ayaka Hagita-Tatsumoto^1,2, Tomohiro Miyasaka^1,2,3

¹Department of Neuropathology, Faculty of Life and Medical Sciences,Doshisha University, ²Center for Research in Neurodegenerative Diseases,Doshisha University, ³Laboratory of Physiology and Anatomy, School of Pharmacy,Nihon University

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