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JOVE JOURNAL
Biochemistry
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Showing 97 - 108 in 822 videos.
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69
Journal (Biochemistry)
Transformation of
E. coli
Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity
Hloniphani Rachel Ncube
1
,
Unarine Dali
1
,
Tendamudzimu Harmfree Dongola
1
,
Addmore Shonhai
1
1
Department of Biochemistry & Microbiology
,
University of Venda
Author
Hloniphani Rachel Ncube
1
,
Unarine Dali
1
,
Tendamudzimu Harmfree Dongola
1
,
Addmore Shonhai
1
Institution
1
Department of Biochemistry & Microbiology
,
University of Venda
AUTHORS
Journal (Biochemistry)
Analysis of Hsp70 Chaperone Activity Using SDS PAGE and Western Blot Analyses of Transformed
E. coli
Protein
Hloniphani Rachel Ncube
1
,
Unarine Dali
1
,
Tendamudzimu Harmfree Dongola
1
,
Addmore Shonhai
1
1
Department of Biochemistry & Microbiology
,
University of Venda
Author
Hloniphani Rachel Ncube
1
,
Unarine Dali
1
,
Tendamudzimu Harmfree Dongola
1
,
Addmore Shonhai
1
Institution
1
Department of Biochemistry & Microbiology
,
University of Venda
AUTHORS
Journal (Biochemistry)
Escherichia coli
-Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70
Hloniphani Rachel Ncube
1
,
Unarine Dali
1
,
Tendamudzimu Harmfree Dongola
1
,
Addmore Shonhai
1
1
Department of Biochemistry & Microbiology
,
University of Venda
Author
Hloniphani Rachel Ncube
1
,
Unarine Dali
1
,
Tendamudzimu Harmfree Dongola
1
,
Addmore Shonhai
1
Institution
1
Department of Biochemistry & Microbiology
,
University of Venda
AUTHORS
Journal (Biochemistry)
Crystallization and
In Situ
Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source
James Sandy
1
,
Halina Mikolajek
1,2
,
Amy J. Thompson
1
,
Juan Sanchez-Weatherby
1
,
Michael A Hough
1,2
1
Diamond Light Source Ltd
,
Harwell Science and Innovation Campus
,
2
Research Complex at Harwell
,
Harwell Science and Innovation Campus
Author
James Sandy
1
,
Halina Mikolajek
1,2
,
Amy J. Thompson
1
,
Juan Sanchez-Weatherby
1
,
Michael A Hough
1,2
Institution
1
Diamond Light Source Ltd
,
Harwell Science and Innovation Campus
,
2
Research Complex at Harwell
,
Harwell Science and Innovation Campus
AUTHORS
Journal (Biochemistry)
In Situ X-ray Crystallographic Data Collection Using the Beamline at Diamond Light Source
James Sandy
1
,
Halina Mikolajek
1,2
,
Amy J. Thompson
1
,
Juan Sanchez-Weatherby
1
,
Michael A Hough
1,2
1
Diamond Light Source Ltd
,
Harwell Science and Innovation Campus
,
2
Research Complex at Harwell
,
Harwell Science and Innovation Campus
Author
James Sandy
1
,
Halina Mikolajek
1,2
,
Amy J. Thompson
1
,
Juan Sanchez-Weatherby
1
,
Michael A Hough
1,2
Institution
1
Diamond Light Source Ltd
,
Harwell Science and Innovation Campus
,
2
Research Complex at Harwell
,
Harwell Science and Innovation Campus
AUTHORS
Journal (Biochemistry)
Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs
Russell Spencer-Smith
1,2,3
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
Hollings Cancer Center
,
Medical University of South Carolina
,
3
Laboratory of Cell and Developmental Signaling, Center for Cancer Research
,
National Cancer Institute-Frederick
Author
Russell Spencer-Smith
1,2,3
Institution
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
Hollings Cancer Center
,
Medical University of South Carolina
,
3
Laboratory of Cell and Developmental Signaling, Center for Cancer Research
,
National Cancer Institute-Frederick
AUTHORS
Journal (Biochemistry)
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
Russell Spencer-Smith
1,2,3
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
Hollings Cancer Center
,
Medical University of South Carolina
,
3
Laboratory of Cell and Developmental Signaling, Center for Cancer Research
,
National Cancer Institute-Frederick
Author
Russell Spencer-Smith
1,2,3
Institution
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
Hollings Cancer Center
,
Medical University of South Carolina
,
3
Laboratory of Cell and Developmental Signaling, Center for Cancer Research
,
National Cancer Institute-Frederick
AUTHORS
Journal (Biochemistry)
Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay
Russell Spencer-Smith
1,2,3
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
Hollings Cancer Center
,
Medical University of South Carolina
,
3
Laboratory of Cell and Developmental Signaling, Center for Cancer Research
,
National Cancer Institute-Frederick
Author
Russell Spencer-Smith
1,2,3
Institution
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
Hollings Cancer Center
,
Medical University of South Carolina
,
3
Laboratory of Cell and Developmental Signaling, Center for Cancer Research
,
National Cancer Institute-Frederick
AUTHORS
Journal (Biochemistry)
DNAzyme 10-23 – Based Nanomachines for Nucleic Acid Recognition
Pavel V. Filatov
1
,
Muhannad Ateiah
1
,
Maria Y. Berezovskaya
1
,
Maria S. Rubel
1
,
Dmitry M. Kolpashchikov
2
1
ITMO University
,
2
University of Central Florida
Author
Pavel V. Filatov
1
,
Muhannad Ateiah
1
,
Maria Y. Berezovskaya
1
,
Maria S. Rubel
1
,
Dmitry M. Kolpashchikov
2
Institution
1
ITMO University
,
2
University of Central Florida
AUTHORS
Journal (Biochemistry)
Designing DNAzyme-Based Nanomachines with a 10-23 Core for Detection of an Epstein-Barr Virus Fragment
Pavel V. Filatov
1
,
Muhannad Ateiah
1
,
Maria Y. Berezovskaya
1
,
Maria S. Rubel
1
,
Dmitry M. Kolpashchikov
2
1
ITMO University
,
2
University of Central Florida
Author
Pavel V. Filatov
1
,
Muhannad Ateiah
1
,
Maria Y. Berezovskaya
1
,
Maria S. Rubel
1
,
Dmitry M. Kolpashchikov
2
Institution
1
ITMO University
,
2
University of Central Florida
AUTHORS
Journal (Biochemistry)
Assembly of DNAzyme-Based Nanomachines for its Functional Characterization
Pavel V. Filatov
1
,
Muhannad Ateiah
1
,
Maria Y. Berezovskaya
1
,
Maria S. Rubel
1
,
Dmitry M. Kolpashchikov
2
1
ITMO University
,
2
University of Central Florida
Author
Pavel V. Filatov
1
,
Muhannad Ateiah
1
,
Maria Y. Berezovskaya
1
,
Maria S. Rubel
1
,
Dmitry M. Kolpashchikov
2
Institution
1
ITMO University
,
2
University of Central Florida
AUTHORS
Journal (Biochemistry)
Limit of Detection (LOD) Assay to Check the Sensitivity of DNAzyme-Based Nanomachines (DNM)
Pavel V. Filatov
1
,
Muhannad Ateiah
1
,
Maria Y. Berezovskaya
1
,
Maria S. Rubel
1
,
Dmitry M. Kolpashchikov
2
1
ITMO University
,
2
University of Central Florida
Author
Pavel V. Filatov
1
,
Muhannad Ateiah
1
,
Maria Y. Berezovskaya
1
,
Maria S. Rubel
1
,
Dmitry M. Kolpashchikov
2
Institution
1
ITMO University
,
2
University of Central Florida
AUTHORS
1
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