JOVE JOURNAL

Developmental Biology

Showing 25 - 36 in 842 videos.

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

Elizabeth Abraham¹, Mikel Zubillaga¹, Thomas Roule², Eleonora Stronati³, Naiara Akizu², Conchi Estaras¹

¹Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center,Temple University, Lewis Katz School of Medicine ²Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children's Hospital of Philadelphia ³Department of Child and Adolescence Psychiatry,Children's Hospital of Philadelphia

Journal (Developmental Biology)

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program,Severo Ochoa Center for Molecular Biology (CBMSO)²Cardiovascular Regeneration Program,National Cardiovascular Research Center (CNIC)³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV)⁴Department of Experimental Biology, Faculty of Experimental Sciences,University of Jaén

Journal (Developmental Biology)

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

Jianan Tang^1,2,3, Baoli Zhang^1,2,3, Chunhua Tang^4,5,6, Xuemei Wang^1,2,3, Minmin Hua^1,2,3, Yuchuan Zhou^4,5,6, Huijuan Shi^1,2,3, Tiancheng Zhang^1,2,3

¹Shanghai-MOST Key Laboratory of Health and Disease Genomics ²NHC Key Lab of Reproduction Regulation ³Shanghai Institute for Biomedical and Pharmaceutical Technologies ⁴International Peace Maternity and Child Health Hospital ⁵School of Medicine,Shanghai Jiao Tong University ⁶Shanghai Key Laboratory of Embryo Original Diseases

Journal (Developmental Biology)

Methods to Enable Spatial Transcriptomics of Bone Tissues

Luigi Mancinelli^1,2, Karen Elizabeth Schoedel^3,4, Kurt Richard Weiss^5,6,7, Giuseppe Intini^1,2,8,9,10

¹Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine ²Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine ³UPMC Presbyterian ⁴UPMC Shadyside ⁵Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh ⁶UPMC Hillman Cancer Center ⁷Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh ⁸Department of Medicine,University of Pittsburgh School of Medicine ⁹University of Pittsburgh UPMC Hillman Cancer Center ¹⁰McGowan Institute for Regenerative Medicine,University of Pittsburgh

Journal (Developmental Biology)

Generation, Maintenance, and Identification of Germ-Free Zebrafish Models from Larvae to Juvenile Stages

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

Swimming Exercise Protocol and Care Methods for Pregnant Rats

Ziqi Ni*¹, Jiaqi Cao*¹, Meiling Shan¹, Yanyan Zhang^1,2,3, Lijun Shi^1,2,3

¹Department of Exercise Physiology,Beijing Sport University ²Laboratory of Sports Stress and Adaptation of General Administration of Sport,Beijing Sport University ³Key Laboratory of Physical Fitness and Exercise, Ministry of Education,Beijing Sport University

Journal (Developmental Biology)

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic^1,3, Anastasiya Boltengagen¹, Agnieszka Rybak-Wolf^1,3, Nikolaus Rajewsky^1,2

¹Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)²Institut für Biologie,Humboldt-Universität zu Berlin ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)

Journal (Developmental Biology)

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Alexander Blümke^1,2, Jessica Simon¹, Elizabeth Leber¹, Marta Scatena¹, Cecilia M. Giachelli¹

¹Department of Bioengineering, Department of Medicine,University of Washington ²Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Journal (Developmental Biology)

Nuclear Isolation from Cryopreserved In Vitro Derived Blood Cells

Rong Qiu¹, Chayanne Petit¹, Christopher S. Thom^1,2

¹Division of Neonatology,Children’s Hospital of Philadelphia ²Department of Pediatrics,University of Pennsylvania Perelman School of Medicine

Journal (Developmental Biology)

Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research

Yuefeng Li^1,2, Xiaofen Wu^1,2, Ping Hu^1,2

¹Guangzhou Laboratory ²The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes,The Fifth Affiliated Hospital of Guangzhou Medical University

Journal (Developmental Biology)

Early Unguided Human Brain Organoid Neurovascular Niche Modeling into the Permissive Chick Embryo Chorioallantoic Membrane

Luciano Fiore^1,2, Jan Arderiu³, Andrea Martí-Sarrias^3,4, Isabel Turpín^3,4, Ruth I. Pareja^3,5, Arcadi Navarro^5,6,7,8, Mariana Holubiec^2,9, Julieta Bianchelli⁹, Tomas Falzone^2,9, Gonzalo Spelzini^1,2, Gabriel Scicolone^1,2, Sandra Acosta^3,4

¹Instituto de Biología Celular y Neurociencias “Prof. E. De Robertis” (IBCN),CONICET - Universidad de Buenos Aires ²Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética,Universidad de Buenos Aires ³Institute of Neurosciences, Pathology and Experimental Therapeutics Dept,University of Barcelona ⁴Functional Neurogenomics Group, Neurodevelopmental Disorders,IDIBELL, L’Hospitalet de Llobregat ⁵IBE, Institute of Evolutionary Biology (UPF-CSIC), Department of Medicine and Life Sciences,Universitat Pompeu Fabra ⁶Institució Catalana de Recerca i Estudis Avançats (ICREA) and Universitat Pompeu Fabra ⁷Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology ⁸BarcelonaBeta Brain Research Center,Pasqual Maragall Foundation ⁹Instituto de Investigación en Biomedicina (IBioBA) – CONICET – Instituto Partner de la Sociedad Max Planck

Journal (Developmental Biology)

Mouse Round Spermatid Injection

Haibo Zhu^1,2, Yuting Jiang^1,2, Haiwei Quan^1,2, Leyi Li^1,2, Jiarui Wei^1,2, Ruizhi Liu^1,2, Ruixue Wang^1,2

¹Center of Reproductive Medicine,First Hospital of Jilin University ²Center of Prenatal Diagnosis,First Hospital of Jilin University

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