JOVE JOURNAL

Developmental Biology

Showing 37 - 48 in 830 videos.

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Cell Dissociation of Isolated Gastrulating Murine Embryos for Single-Cell Sequencing

Journal (Developmental Biology)

Cell Dissociation of Isolated Gastrulating Murine Embryos for Single-Cell Sequencing

Elizabeth Abraham¹, Mikel Zubillaga¹, Thomas Roule², Eleonora Stronati³, Naiara Akizu², Conchi Estaras¹

¹Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center,Temple University, Lewis Katz School of Medicine, ²Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children's Hospital of Philadelphia, ³Department of Child and Adolescence Psychiatry,Children's Hospital of Philadelphia

Extraction and Electroporation of Mouse Embryo Heart as a Transgenesis Approach

Journal (Developmental Biology)

Extraction and Electroporation of Mouse Embryo Heart as a Transgenesis Approach

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program,Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program,National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences,University of Jaén

Electroporated Mouse Cardiac Cell Isolation for Cell Sorting and Analysis

Journal (Developmental Biology)

Electroporated Mouse Cardiac Cell Isolation for Cell Sorting and Analysis

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program,Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program,National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences,University of Jaén

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Journal (Developmental Biology)

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program,Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program,National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences,University of Jaén

Intracytoplasmic Sperm Injection (ICSI) and Metabolic Assessments in Mice

Journal (Developmental Biology)

Intracytoplasmic Sperm Injection (ICSI) and Metabolic Assessments in Mice

Jianan Tang^1,2,3, Baoli Zhang^1,2,3, Chunhua Tang^4,5,6, Xuemei Wang^1,2,3, Minmin Hua^1,2,3, Yuchuan Zhou^4,5,6, Huijuan Shi^1,2,3, Tiancheng Zhang^1,2,3

¹Shanghai-MOST Key Laboratory of Health and Disease Genomics, ²NHC Key Lab of Reproduction Regulation, ³Shanghai Institute for Biomedical and Pharmaceutical Technologies, ⁴International Peace Maternity and Child Health Hospital, ⁵School of Medicine,Shanghai Jiao Tong University, ⁶Shanghai Key Laboratory of Embryo Original Diseases

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

Journal (Developmental Biology)

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

Jianan Tang^1,2,3, Baoli Zhang^1,2,3, Chunhua Tang^4,5,6, Xuemei Wang^1,2,3, Minmin Hua^1,2,3, Yuchuan Zhou^4,5,6, Huijuan Shi^1,2,3, Tiancheng Zhang^1,2,3

¹Shanghai-MOST Key Laboratory of Health and Disease Genomics, ²NHC Key Lab of Reproduction Regulation, ³Shanghai Institute for Biomedical and Pharmaceutical Technologies, ⁴International Peace Maternity and Child Health Hospital, ⁵School of Medicine,Shanghai Jiao Tong University, ⁶Shanghai Key Laboratory of Embryo Original Diseases

Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies

Journal (Developmental Biology)

Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies

Luigi Mancinelli^1,2, Karen Elizabeth Schoedel^3,4, Kurt Richard Weiss^5,6,7, Giuseppe Intini^1,2,8,9,10

¹Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine, ²Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine, ³UPMC Presbyterian, ⁴UPMC Shadyside, ⁵Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh, ⁶UPMC Hillman Cancer Center, ⁷Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh, ⁸Department of Medicine,University of Pittsburgh School of Medicine, ⁹University of Pittsburgh UPMC Hillman Cancer Center, ¹⁰McGowan Institute for Regenerative Medicine,University of Pittsburgh

Methods to Enable Spatial Transcriptomics of Bone Tissues

Journal (Developmental Biology)

Methods to Enable Spatial Transcriptomics of Bone Tissues

Luigi Mancinelli^1,2, Karen Elizabeth Schoedel^3,4, Kurt Richard Weiss^5,6,7, Giuseppe Intini^1,2,8,9,10

¹Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine, ²Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine, ³UPMC Presbyterian, ⁴UPMC Shadyside, ⁵Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh, ⁶UPMC Hillman Cancer Center, ⁷Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh, ⁸Department of Medicine,University of Pittsburgh School of Medicine, ⁹University of Pittsburgh UPMC Hillman Cancer Center, ¹⁰McGowan Institute for Regenerative Medicine,University of Pittsburgh

Generation of Germ-Free Zebrafish Models from Embryos

Journal (Developmental Biology)

Generation of Germ-Free Zebrafish Models from Embryos

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

GF Artemia nauplii Preparation for Chronic GF Zebrafish Nutrition

Journal (Developmental Biology)

GF Artemia nauplii Preparation for Chronic GF Zebrafish Nutrition

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

Generation, Maintenance, and Identification of Germ-Free Zebrafish Models from Larvae to Juvenile Stages

Journal (Developmental Biology)

Generation, Maintenance, and Identification of Germ-Free Zebrafish Models from Larvae to Juvenile Stages

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

Tracking Germ-Free Zebrafish Models Across All Life Stages

Journal (Developmental Biology)

Tracking Germ-Free Zebrafish Models Across All Life Stages

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

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