JOVE JOURNAL

Developmental Biology

Showing 61 - 72 in 830 videos.

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

Carla Guzman-Moreno*¹, Peizhao Zhang*², Olivia R. Phillips*¹, Mathias Block*³, Benjamin J. Glennon*¹, Meghan Holbrook², Lauren Weigand², Cecilia W. Lo¹, Jiuann-Huey I. Lin^1,4

¹Department of Developmental Biology,University of Pittsburgh, ²Department of Biological Sciences,University of Pittsburgh, ³Département de Biologie,École Normale Supérieure de Lyon, ⁴Department of Critical Care Medicine,University of Pittsburgh

Journal (Developmental Biology)

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Chantal K. Diallo¹, Andrew J. Modzelewski¹

¹Department of Biomedical Sciences, School of Veterinary Medicine,The University of Pennsylvania

Journal (Developmental Biology)

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Sudipta Mahato*^1,2, Trupti Agrawal*^1,2, Divya Pidishetty*^1,2, Savitri Maddileti¹, Vinay Kumar Pulimamidi^1,3, Indumathi Mariappan¹

¹Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre,LV Prasad Eye Institute, ²Manipal Academy of Higher Education, ³Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School

Journal (Developmental Biology)

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

John R. Klem¹, Raèden Gray¹, C. Ben Lovely¹

¹Alcohol Research Center, Department of Biochemistry and Molecular Genetics,University of Louisville

Journal (Developmental Biology)

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Daniela Londoño-Vásquez^1,2, Alexander Jurkevich³, Ahmed Z. Balboula¹

¹Animal Sciences Research Center,University of Missouri, ²Universidad Nacional de Colombia, ³Advanced Light Microscopy Core,University of Missouri

Journal (Developmental Biology)

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

Aishwarya Swaminathan¹, Yung Hwang¹, Ashley Winward¹, Merav Socolovsky¹

¹Department of Molecular, Cell and Cancer Biology,UMASS Chan Medical School

Journal (Developmental Biology)

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Mansour Aboelenain^1,2,3, Karen Schindler^1,2, Cecilia S. Blengini^1,2

¹Department of Genetics,Rutgers, The State University of New Jersey, ²Human Genetics Institute of New Jersey, ³Department of Theriogenology, Faculty of Veterinary Medicine,Mansoura University

Journal (Developmental Biology)

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

Yixuan Feng^1,2, Tai Wang^1,2,3, Lingtong Liu¹

¹Key Laboratory of Plant Molecular Physiology, Institute of Botany,Chinese Academy of Sciences, ²College of Life Science,University of Chinese Academy of Sciences, ³Innovative Academy of Seed Design,Chinese Academy of Sciences

Journal (Developmental Biology)

Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage

Jayesh Kumar Sevak¹, Suchitra D. Gopinath¹

¹Translational Health Science and Technology Institute (THSTI),NCR Biotech Science Cluster

Journal (Developmental Biology)

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Dina H. Kassem¹, Sarah A. Habib², Omar I. Badr², Mohamed M. Kamal^1,2,3

¹Biochemistry Department, Faculty of Pharmacy,Ain Shams University, ²Pharmacology and Biochemistry Department, Faculty of Pharmacy,The British University in Egypt, ³Center for Drug Research and Development (CDRD), Faculty of Pharmacy,The British University in Egypt

Journal (Developmental Biology)

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Manabu Ozawa*¹, Chihiro Emori*², Masahito Ikawa^1,2

¹Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science,The University of Tokyo, ²Research Institute for Microbial Diseases,Osaka University

Journal (Developmental Biology)

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Sarah Avila-Barnard¹, David C. Volz¹

¹Department of Environmental Sciences,University of California, Riverside

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