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January 05, 2016
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Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.
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Konadu, K. A., Huang, M. B., Roth, W., Armstrong, W., Powell, M., Villinger, F., Bond, V. Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals. J. Vis. Exp. (107), e53495, doi:10.3791/53495 (2016).
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