Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Chapitre 4
Domaine de liaison du ligand
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their…
Interfaces protéines-protéines
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most…
Domaine de liaison conservé
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein…
Constante de liaison à l'équilibre et force de liaison
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is…
Cofacteurs et coenzymes
Enzymes require additional components for proper function. There are two such classes of molecules: cofactors and coenzymes. Cofactors are metallic…
Régulation allostérique
Allosteric regulation of enzymes occurs when the binding of a molecule to a different location from the active site causes a change in enzymatic…
Liaison et liaison-ligand
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the…
Allosteric Proteins-ATCase
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism…
Transitions allostériques coopératives
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand…
Phosphorylation
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These…
Protéine kinases et phosphatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation,…
Les GTPases et leur régulation
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as…
Régulateurs de protéines à liaison covalente
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an…
Complexes protéiques avec parties interchangeables
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s…
Fonctions des protéines mécaniques
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical…
Fonction des protéines structurales
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major…
Réseaux protéiques
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other…
Assaying Protein Kinase Activity with Radiolabeled ATP
Protein kinases are able to govern large-scale cellular changes in response to complex arrays of stimuli, and much effort has been directed at…
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
Proteins are an important class of biological macromolecules that play many key roles in cellular functions including gene expression, catalyzing…
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
Dynamic protein-protein interactions control cellular behavior, from motility to DNA replication to signal transduction. However, monitoring dynamic…

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter