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Visão Geral do Reparo de DNA
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand…
Reparo de Excisão de Base
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing…
Reparo de Excisão de Base de Faixa Longa
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining…
Reparação da Excisão de Nucleótidos
Exposure to mutagens can damage DNA and result in bulky lesions that distort the double-helix structure or impede proper transcription. Damaged DNA…
Polimerases de Translesão de DNA
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a…
Reparando Quebras de Fita dupla
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves…
Danos no DNA podem Paralisar o Ciclo Celular
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical…
Recombinação Homóloga
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One…
Reiniciando Garfos de Replicação Paralisados
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the…
Conversão de Gene
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the…
Visão Geral da Transposição e Recombinação
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role…
Transpósons apenas de DNA
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism.…
Retrovírus
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on…
Retrotranspósons LTR
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less…
Retrotranspósons não LTR
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and…
Recombinação Específica do Local Conservador e Variação de Fase
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic…
Microscopia de Força Atômica Investigações do Reconhecimento de Lesão de DNA no Reparo de Excisão de Nucleótidos
AFM imaging is a powerful technique for the study of protein-DNA interactions. This single molecule method allows the simultaneous resolution of…
Observação em tempo real da reação de troca de Strand DNA mediada por Rad51
The DNA strand exchange reaction mediated by Rad51 is a critical step of homologous recombination. In this reaction, Rad51 forms a nucleoprotein…
Sequenciamento da próxima geração de RNA e um pipeline de Bioinformática para identificar a linha-1s expressa no nível específico do locus
Long INterspersed Elements-1 (LINEs/L1s) are repetitive elements that can copy and randomly insert in the genome resulting in genomic instability and…

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