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Capítulo 13
La hélice de ADN
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most…
Empaquetamiento del ADN
Eukaryotes have large genomes compared to prokaryotes. In order to fit their genomes into a cell, eukaryotes must pack their DNA tightly inside the…
Organización de genes
The genomes of eukaryotes can be structured in several functional categories. A strand of DNA is comprised of genes and intergenic regions. Genes…
Cariotipo
Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated…
Replicación en procariotas
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to…
Replicación en eucariotas
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity…
Corrección de pruebas
Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA…
Reparación de discordancias
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires…
Reparación de escisión de nucleótidos
Exposure to mutagens can damage DNA and result in bulky lesions that distort the double-helix structure or impede proper transcription. Damaged DNA…
Mutaciones
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors.…
Transcripción
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene…
Traducción
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it…
Transformación Bacteriana
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied…
Plegable y caracterización de un Robot Bio-sensible de ADN Origami
The DNA nanorobot is a hollow hexagonal nanometric device, designed to open in response to specific stimuli and present cargo sequestered inside.…
El Método chip-exo: La identificación de las interacciones proteína-ADN con zona de Base Par de precisión
Chromatin immunoprecipitation (ChIP) is an indispensable tool in the fields of epigenetics and gene regulation that isolates specific protein-DNA…
Aplicación de métodos de flujo detenido Cinética para investigar el mecanismo de acción de una proteína de reparación del ADN
Transient kinetic analysis is indispensable for understanding the workings of biological macromolecules, since this approach yields mechanistic…
Investigaciones de Microscopía de Fuerza Atómica de Reconocimiento de Lesión de ADN en Reparación de Excisión de Nucleótidos
AFM imaging is a powerful technique for the study of protein-DNA interactions. This single molecule method allows the simultaneous resolution of…

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