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Capítulo 15
O que é a Engenharia Genética?
Genetic engineering is the process of modifying an organism’s DNA to introduce new, desirable traits. Many organisms, from bacteria to plants…
Seleção de Antibióticos
Researchers use antibiotic resistance genes to identify bacteria that possess a plasmid containing their gene of interest. Antibiotic resistance…
DNA Recombinante
Scientists create recombinant DNA by combining DNA from different sources—often, other species—in the laboratory. DNA cloning allows…
Organismos Transgénicos
Transgenic organisms are genetically engineered to carry transgenes—genes from a different species—as part of their genome. The…
Células Estaminais Adultas
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell…
Células Estaminais Embrionárias
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous…
Células Estaminais Pluripotentes Induzidas
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific…
Mutagénese In-vitro
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by…
Isolamento do DNA
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells,…
Terapia Genética
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a…
Clonagem Reprodutiva
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced…
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at…
DNA Complementar
Only genes that are transcribed into messenger RNA (mRNA) are active, or expressed. Scientists can, therefore, extract the mRNA from cells to study…
PCR
The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce…
Genómica
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried…
Culturas alternativas para pluripotentes estaminais humanas produção de células, Manutenção e Análise Genética
Human pluripotent stem cells (hPSCs) hold great promise for regenerative medicine and biopharmaceutical applications. Currently, optimal culture and…
Direcionado a próxima geração sequenciamento e bioinformática da Pipeline para avaliar determinantes genéticos da doença constitucional
Next-generation sequencing (NGS) is quickly revolutionizing how research into the genetic determinants of constitutional disease is performed. The…
O Chip Multi-organ - Uma plataforma microfluídica para longo prazo Coculture Multi-tecidos
The ever growing amount of new substances released onto the market and the limited predictability of current in vitro test systems has led to a high…
CRISPR guia RNA clonagem para sistemas de mamíferos
The outlined protocol describes streamlined methods for the efficient and cost-effective generation of Cas9-associated guide RNAs. Two alternative…
CAPRRESI: montagem de quimera por recuperação de plasmídeos e restrição de inserção do site enzimático
Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI). CAPRRESI benefits from many strengths of the…
Síntese de nanofibras baseado em queratina de Engenharia Biomédica
Electrospinning, due to its versatility and potential for applications in various fields, is being frequently used to fabricate nanofibers.…
Protocolos para Avaliação de radiofreqüência Interações com Nanopartículas de Ouro e Sistemas biológicos para terapia não-invasiva Câncer Hipertermia
Cancer therapies which are less toxic and invasive than their existing counterparts are highly desirable. The use of RF electric-fields that…

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