Jove Lab Bio

Lab 11: Bacterial Transformation — Materials List

Preparation for 10 Students

• pGreen Plasmid (0.005 µg/µL)
  10 µL
• E. coli (OP50 strain) (1 tube)
  5 tubes
• 1M CaCl2
  5 mL
• LB broth media
  500 mL
• LB+AMP agar plates (100µg/mL AMP)
  10
• LB agar plates
  20
• SOC media
  10 mL
• Goggles
  5
• Inoculating loops
  5
• Laboratory film/ Parafilm
  1 per station
• 70% Ethanol spray bottle
  1 per station
• Microfuge tubes (1.5mL)
  1 full beaker/station
• Pipettes (1- 1000µL)
  1 set/station
• Pipett tips (tip boxes) (1- 1000µL)
  1 set/station
• Incubator (Set to 37°C)
  Dependent on the lab size
• Water bath (Set to 42°C)
  Dependent on the lab size
• UV Transilluminator
  Dependent on the lab size